![]() ![]() In the proposed model, the formation of protein–protein interfaces is thought to be driven by hydrophobic effects, involving a reorganization of the well-ordered water structure on the hydrophobic surfaces of the protein–protein complex. In this work, based on similarities between protein precipitation and HIC, a new precipitation model was derived. Lately, a new isotherm for hydrophobic interaction chromatography (HIC) under consideration of water molecules as participants was proposed, enabling an accurate description of HIC. In preparative chromatography, mechanistic modeling was successfully shown for a variety of applications. Mechanistic modeling can be a means for faster, material-saving process development and a better process understanding at the same time. One reason might be that the mechanism behind protein phase behavior is not completely understood and the precipitation process development is still empirical. Although the capability has been proven, there has been no precipitation process realized for large-scale monoclonal antibody (mAb) production yet. ![]() Selective preparative protein precipitation is one promising alternative technique. Especially with increasing product titers, drawbacks like slow volumetric throughput and high resin costs lead to an intensifying need for alternative technologies. For the purification of biopharmaceutical proteins, liquidĬhromatography is still the gold standard. ![]()
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